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201192a maxpar cell staining buffer fluidigm  (fluidigm)


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    fluidigm 201192a maxpar cell staining buffer fluidigm
    201192a Maxpar Cell Staining Buffer Fluidigm, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 1486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/201192a+maxpar+cell+staining+buffer/Cell-ID+Intercalator-Ir/pm39672166-626-156-161
    Average 96 stars, based on 1486 article reviews
    201192a maxpar cell staining buffer fluidigm - by Bioz Stars, 2026-09
    96/100 stars

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    Transfection:

    Article Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P/S Fisher Scientific Cat. #11548876 Polyethylenimine Polysciences Cat. #23966 Fugene HD transfection Promega Cat. #E2311 Puromycin InvivoGen Cat. #ant-pr-1 SCH-58261 Sigma-Aldrich Cat. #S4568-5MG PMA Sigma-Aldrich Cat. #10634-1MG Ionomycin Sigma-Aldrich Cat. #10634-1MG DMSO Sigma-Aldrich Cat. #D2650-100ML Ficoll-Hypaque Corning Cat. #25-072-Cl aCD3/aCD28 activating beads ThermoFisher Scientific Cat. #11132D IL-2 PeproTech Cat. #200-02 DMOG Sigma-Aldrich Cat. #D3695 Saquinavir (Protease Inhibitor) Sigma-Aldrich Cat. #S8451-50MG Raltegravir (Integrase Inhibitor) Sigma-Aldrich Cat. #CDS023737-25MG Contaminant-free PBS (for CyTOF experiments) Rockland Cat. #MB-008 EDTA (for CyTOF experiments) Corning Cat. # 46-034-CI Barcode Perm buffer Fluidigm Cat. # 201057 PFA (for CyTOF experiments) Electron Microscopy Sciences Cat. #15700 fix/perm buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #88-8824-00 Permeabilization Buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #00-8333-56 Cell-ID DNA Intercalator-Ir (for CyTOF experiments) Fluidigm Cat. #201192A Maxpar Cell Staining Buffer (for CyTOF experiments) Fluidigm Cat. # 201068 Maxpar PBS (for CyTOF experiments) Fluidigm Cat. #201058 Maxpar Cell Acquisition Solution (for CyTOF experiments) Fluidigm Cat. #201240 EQ calibration beads (for CyTOF experiments) Fluidigm Cat. #201078 retrieval solution Agilent Dako Cat. #S1700 Triton X-100 Sigma-Aldrich Cat. #X100 Zombie viability dye Biolegend Cat. #423113 live/dead staining ThermoFisher Scientific Cat. #L34961 Prolong Diamond Antifade Mount medium containing DAPI ThermoFisher Scientific Cat. #P36931 Cisplatin Sigma-Aldrich Cat. #232120-50MG Critical commercial assays NucleoSpin Plasmid, Mini kit Macherey-Nagel Cat. #740588 Plasmid Plus Maxi Kit Qiagen Cat. #12963 Quick-RNA Miniprep Kit Zymo Research Cat. #R1054 HIV-1 alliance p24 ELISA kit PerkinElmer Cat. #NEK050001KT EasySep Human CD4+ T cell Isolation Cocktail StemCell Technologies Cat. #17952 luciferase assay system Promega Cat. #E2610 nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay NanoString Technologies Cat. #121100019 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat. # 201060 PNA ISH kit Agilent Dako Cat. #K5201 RNAscope 2.5 HD Detection Reagent-RED Advanced Cell Diagnostics Cat. #322360 Deposited data NanoString nCounter data, raw and analyzed This paper GEO: GSE244193 RNA sequencing data This paper GEO: GSE244193 (Continued on next page) 18 Cell Reports 42, 113285, November 28, 2023

    Protease Inhibitor:

    Article Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P/S Fisher Scientific Cat. #11548876 Polyethylenimine Polysciences Cat. #23966 Fugene HD transfection Promega Cat. #E2311 Puromycin InvivoGen Cat. #ant-pr-1 SCH-58261 Sigma-Aldrich Cat. #S4568-5MG PMA Sigma-Aldrich Cat. #10634-1MG Ionomycin Sigma-Aldrich Cat. #10634-1MG DMSO Sigma-Aldrich Cat. #D2650-100ML Ficoll-Hypaque Corning Cat. #25-072-Cl aCD3/aCD28 activating beads ThermoFisher Scientific Cat. #11132D IL-2 PeproTech Cat. #200-02 DMOG Sigma-Aldrich Cat. #D3695 Saquinavir (Protease Inhibitor) Sigma-Aldrich Cat. #S8451-50MG Raltegravir (Integrase Inhibitor) Sigma-Aldrich Cat. #CDS023737-25MG Contaminant-free PBS (for CyTOF experiments) Rockland Cat. #MB-008 EDTA (for CyTOF experiments) Corning Cat. # 46-034-CI Barcode Perm buffer Fluidigm Cat. # 201057 PFA (for CyTOF experiments) Electron Microscopy Sciences Cat. #15700 fix/perm buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #88-8824-00 Permeabilization Buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #00-8333-56 Cell-ID DNA Intercalator-Ir (for CyTOF experiments) Fluidigm Cat. #201192A Maxpar Cell Staining Buffer (for CyTOF experiments) Fluidigm Cat. # 201068 Maxpar PBS (for CyTOF experiments) Fluidigm Cat. #201058 Maxpar Cell Acquisition Solution (for CyTOF experiments) Fluidigm Cat. #201240 EQ calibration beads (for CyTOF experiments) Fluidigm Cat. #201078 retrieval solution Agilent Dako Cat. #S1700 Triton X-100 Sigma-Aldrich Cat. #X100 Zombie viability dye Biolegend Cat. #423113 live/dead staining ThermoFisher Scientific Cat. #L34961 Prolong Diamond Antifade Mount medium containing DAPI ThermoFisher Scientific Cat. #P36931 Cisplatin Sigma-Aldrich Cat. #232120-50MG Critical commercial assays NucleoSpin Plasmid, Mini kit Macherey-Nagel Cat. #740588 Plasmid Plus Maxi Kit Qiagen Cat. #12963 Quick-RNA Miniprep Kit Zymo Research Cat. #R1054 HIV-1 alliance p24 ELISA kit PerkinElmer Cat. #NEK050001KT EasySep Human CD4+ T cell Isolation Cocktail StemCell Technologies Cat. #17952 luciferase assay system Promega Cat. #E2610 nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay NanoString Technologies Cat. #121100019 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat. # 201060 PNA ISH kit Agilent Dako Cat. #K5201 RNAscope 2.5 HD Detection Reagent-RED Advanced Cell Diagnostics Cat. #322360 Deposited data NanoString nCounter data, raw and analyzed This paper GEO: GSE244193 RNA sequencing data This paper GEO: GSE244193 (Continued on next page) 18 Cell Reports 42, 113285, November 28, 2023

    Electron Microscopy:

    Article Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P/S Fisher Scientific Cat. #11548876 Polyethylenimine Polysciences Cat. #23966 Fugene HD transfection Promega Cat. #E2311 Puromycin InvivoGen Cat. #ant-pr-1 SCH-58261 Sigma-Aldrich Cat. #S4568-5MG PMA Sigma-Aldrich Cat. #10634-1MG Ionomycin Sigma-Aldrich Cat. #10634-1MG DMSO Sigma-Aldrich Cat. #D2650-100ML Ficoll-Hypaque Corning Cat. #25-072-Cl aCD3/aCD28 activating beads ThermoFisher Scientific Cat. #11132D IL-2 PeproTech Cat. #200-02 DMOG Sigma-Aldrich Cat. #D3695 Saquinavir (Protease Inhibitor) Sigma-Aldrich Cat. #S8451-50MG Raltegravir (Integrase Inhibitor) Sigma-Aldrich Cat. #CDS023737-25MG Contaminant-free PBS (for CyTOF experiments) Rockland Cat. #MB-008 EDTA (for CyTOF experiments) Corning Cat. # 46-034-CI Barcode Perm buffer Fluidigm Cat. # 201057 PFA (for CyTOF experiments) Electron Microscopy Sciences Cat. #15700 fix/perm buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #88-8824-00 Permeabilization Buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #00-8333-56 Cell-ID DNA Intercalator-Ir (for CyTOF experiments) Fluidigm Cat. #201192A Maxpar Cell Staining Buffer (for CyTOF experiments) Fluidigm Cat. # 201068 Maxpar PBS (for CyTOF experiments) Fluidigm Cat. #201058 Maxpar Cell Acquisition Solution (for CyTOF experiments) Fluidigm Cat. #201240 EQ calibration beads (for CyTOF experiments) Fluidigm Cat. #201078 retrieval solution Agilent Dako Cat. #S1700 Triton X-100 Sigma-Aldrich Cat. #X100 Zombie viability dye Biolegend Cat. #423113 live/dead staining ThermoFisher Scientific Cat. #L34961 Prolong Diamond Antifade Mount medium containing DAPI ThermoFisher Scientific Cat. #P36931 Cisplatin Sigma-Aldrich Cat. #232120-50MG Critical commercial assays NucleoSpin Plasmid, Mini kit Macherey-Nagel Cat. #740588 Plasmid Plus Maxi Kit Qiagen Cat. #12963 Quick-RNA Miniprep Kit Zymo Research Cat. #R1054 HIV-1 alliance p24 ELISA kit PerkinElmer Cat. #NEK050001KT EasySep Human CD4+ T cell Isolation Cocktail StemCell Technologies Cat. #17952 luciferase assay system Promega Cat. #E2610 nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay NanoString Technologies Cat. #121100019 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat. # 201060 PNA ISH kit Agilent Dako Cat. #K5201 RNAscope 2.5 HD Detection Reagent-RED Advanced Cell Diagnostics Cat. #322360 Deposited data NanoString nCounter data, raw and analyzed This paper GEO: GSE244193 RNA sequencing data This paper GEO: GSE244193 (Continued on next page) 18 Cell Reports 42, 113285, November 28, 2023

    Staining:

    Article Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P/S Fisher Scientific Cat. #11548876 Polyethylenimine Polysciences Cat. #23966 Fugene HD transfection Promega Cat. #E2311 Puromycin InvivoGen Cat. #ant-pr-1 SCH-58261 Sigma-Aldrich Cat. #S4568-5MG PMA Sigma-Aldrich Cat. #10634-1MG Ionomycin Sigma-Aldrich Cat. #10634-1MG DMSO Sigma-Aldrich Cat. #D2650-100ML Ficoll-Hypaque Corning Cat. #25-072-Cl aCD3/aCD28 activating beads ThermoFisher Scientific Cat. #11132D IL-2 PeproTech Cat. #200-02 DMOG Sigma-Aldrich Cat. #D3695 Saquinavir (Protease Inhibitor) Sigma-Aldrich Cat. #S8451-50MG Raltegravir (Integrase Inhibitor) Sigma-Aldrich Cat. #CDS023737-25MG Contaminant-free PBS (for CyTOF experiments) Rockland Cat. #MB-008 EDTA (for CyTOF experiments) Corning Cat. # 46-034-CI Barcode Perm buffer Fluidigm Cat. # 201057 PFA (for CyTOF experiments) Electron Microscopy Sciences Cat. #15700 fix/perm buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #88-8824-00 Permeabilization Buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #00-8333-56 Cell-ID DNA Intercalator-Ir (for CyTOF experiments) Fluidigm Cat. #201192A Maxpar Cell Staining Buffer (for CyTOF experiments) Fluidigm Cat. # 201068 Maxpar PBS (for CyTOF experiments) Fluidigm Cat. #201058 Maxpar Cell Acquisition Solution (for CyTOF experiments) Fluidigm Cat. #201240 EQ calibration beads (for CyTOF experiments) Fluidigm Cat. #201078 retrieval solution Agilent Dako Cat. #S1700 Triton X-100 Sigma-Aldrich Cat. #X100 Zombie viability dye Biolegend Cat. #423113 live/dead staining ThermoFisher Scientific Cat. #L34961 Prolong Diamond Antifade Mount medium containing DAPI ThermoFisher Scientific Cat. #P36931 Cisplatin Sigma-Aldrich Cat. #232120-50MG Critical commercial assays NucleoSpin Plasmid, Mini kit Macherey-Nagel Cat. #740588 Plasmid Plus Maxi Kit Qiagen Cat. #12963 Quick-RNA Miniprep Kit Zymo Research Cat. #R1054 HIV-1 alliance p24 ELISA kit PerkinElmer Cat. #NEK050001KT EasySep Human CD4+ T cell Isolation Cocktail StemCell Technologies Cat. #17952 luciferase assay system Promega Cat. #E2610 nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay NanoString Technologies Cat. #121100019 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat. # 201060 PNA ISH kit Agilent Dako Cat. #K5201 RNAscope 2.5 HD Detection Reagent-RED Advanced Cell Diagnostics Cat. #322360 Deposited data NanoString nCounter data, raw and analyzed This paper GEO: GSE244193 RNA sequencing data This paper GEO: GSE244193 (Continued on next page) 18 Cell Reports 42, 113285, November 28, 2023

    Plasmid Preparation:

    Article Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P/S Fisher Scientific Cat. #11548876 Polyethylenimine Polysciences Cat. #23966 Fugene HD transfection Promega Cat. #E2311 Puromycin InvivoGen Cat. #ant-pr-1 SCH-58261 Sigma-Aldrich Cat. #S4568-5MG PMA Sigma-Aldrich Cat. #10634-1MG Ionomycin Sigma-Aldrich Cat. #10634-1MG DMSO Sigma-Aldrich Cat. #D2650-100ML Ficoll-Hypaque Corning Cat. #25-072-Cl aCD3/aCD28 activating beads ThermoFisher Scientific Cat. #11132D IL-2 PeproTech Cat. #200-02 DMOG Sigma-Aldrich Cat. #D3695 Saquinavir (Protease Inhibitor) Sigma-Aldrich Cat. #S8451-50MG Raltegravir (Integrase Inhibitor) Sigma-Aldrich Cat. #CDS023737-25MG Contaminant-free PBS (for CyTOF experiments) Rockland Cat. #MB-008 EDTA (for CyTOF experiments) Corning Cat. # 46-034-CI Barcode Perm buffer Fluidigm Cat. # 201057 PFA (for CyTOF experiments) Electron Microscopy Sciences Cat. #15700 fix/perm buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #88-8824-00 Permeabilization Buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #00-8333-56 Cell-ID DNA Intercalator-Ir (for CyTOF experiments) Fluidigm Cat. #201192A Maxpar Cell Staining Buffer (for CyTOF experiments) Fluidigm Cat. # 201068 Maxpar PBS (for CyTOF experiments) Fluidigm Cat. #201058 Maxpar Cell Acquisition Solution (for CyTOF experiments) Fluidigm Cat. #201240 EQ calibration beads (for CyTOF experiments) Fluidigm Cat. #201078 retrieval solution Agilent Dako Cat. #S1700 Triton X-100 Sigma-Aldrich Cat. #X100 Zombie viability dye Biolegend Cat. #423113 live/dead staining ThermoFisher Scientific Cat. #L34961 Prolong Diamond Antifade Mount medium containing DAPI ThermoFisher Scientific Cat. #P36931 Cisplatin Sigma-Aldrich Cat. #232120-50MG Critical commercial assays NucleoSpin Plasmid, Mini kit Macherey-Nagel Cat. #740588 Plasmid Plus Maxi Kit Qiagen Cat. #12963 Quick-RNA Miniprep Kit Zymo Research Cat. #R1054 HIV-1 alliance p24 ELISA kit PerkinElmer Cat. #NEK050001KT EasySep Human CD4+ T cell Isolation Cocktail StemCell Technologies Cat. #17952 luciferase assay system Promega Cat. #E2610 nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay NanoString Technologies Cat. #121100019 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat. # 201060 PNA ISH kit Agilent Dako Cat. #K5201 RNAscope 2.5 HD Detection Reagent-RED Advanced Cell Diagnostics Cat. #322360 Deposited data NanoString nCounter data, raw and analyzed This paper GEO: GSE244193 RNA sequencing data This paper GEO: GSE244193 (Continued on next page) 18 Cell Reports 42, 113285, November 28, 2023

    Enzyme-linked Immunosorbent Assay:

    Article Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P/S Fisher Scientific Cat. #11548876 Polyethylenimine Polysciences Cat. #23966 Fugene HD transfection Promega Cat. #E2311 Puromycin InvivoGen Cat. #ant-pr-1 SCH-58261 Sigma-Aldrich Cat. #S4568-5MG PMA Sigma-Aldrich Cat. #10634-1MG Ionomycin Sigma-Aldrich Cat. #10634-1MG DMSO Sigma-Aldrich Cat. #D2650-100ML Ficoll-Hypaque Corning Cat. #25-072-Cl aCD3/aCD28 activating beads ThermoFisher Scientific Cat. #11132D IL-2 PeproTech Cat. #200-02 DMOG Sigma-Aldrich Cat. #D3695 Saquinavir (Protease Inhibitor) Sigma-Aldrich Cat. #S8451-50MG Raltegravir (Integrase Inhibitor) Sigma-Aldrich Cat. #CDS023737-25MG Contaminant-free PBS (for CyTOF experiments) Rockland Cat. #MB-008 EDTA (for CyTOF experiments) Corning Cat. # 46-034-CI Barcode Perm buffer Fluidigm Cat. # 201057 PFA (for CyTOF experiments) Electron Microscopy Sciences Cat. #15700 fix/perm buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #88-8824-00 Permeabilization Buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #00-8333-56 Cell-ID DNA Intercalator-Ir (for CyTOF experiments) Fluidigm Cat. #201192A Maxpar Cell Staining Buffer (for CyTOF experiments) Fluidigm Cat. # 201068 Maxpar PBS (for CyTOF experiments) Fluidigm Cat. #201058 Maxpar Cell Acquisition Solution (for CyTOF experiments) Fluidigm Cat. #201240 EQ calibration beads (for CyTOF experiments) Fluidigm Cat. #201078 retrieval solution Agilent Dako Cat. #S1700 Triton X-100 Sigma-Aldrich Cat. #X100 Zombie viability dye Biolegend Cat. #423113 live/dead staining ThermoFisher Scientific Cat. #L34961 Prolong Diamond Antifade Mount medium containing DAPI ThermoFisher Scientific Cat. #P36931 Cisplatin Sigma-Aldrich Cat. #232120-50MG Critical commercial assays NucleoSpin Plasmid, Mini kit Macherey-Nagel Cat. #740588 Plasmid Plus Maxi Kit Qiagen Cat. #12963 Quick-RNA Miniprep Kit Zymo Research Cat. #R1054 HIV-1 alliance p24 ELISA kit PerkinElmer Cat. #NEK050001KT EasySep Human CD4+ T cell Isolation Cocktail StemCell Technologies Cat. #17952 luciferase assay system Promega Cat. #E2610 nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay NanoString Technologies Cat. #121100019 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat. # 201060 PNA ISH kit Agilent Dako Cat. #K5201 RNAscope 2.5 HD Detection Reagent-RED Advanced Cell Diagnostics Cat. #322360 Deposited data NanoString nCounter data, raw and analyzed This paper GEO: GSE244193 RNA sequencing data This paper GEO: GSE244193 (Continued on next page) 18 Cell Reports 42, 113285, November 28, 2023

    Cell Isolation:

    Article Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P/S Fisher Scientific Cat. #11548876 Polyethylenimine Polysciences Cat. #23966 Fugene HD transfection Promega Cat. #E2311 Puromycin InvivoGen Cat. #ant-pr-1 SCH-58261 Sigma-Aldrich Cat. #S4568-5MG PMA Sigma-Aldrich Cat. #10634-1MG Ionomycin Sigma-Aldrich Cat. #10634-1MG DMSO Sigma-Aldrich Cat. #D2650-100ML Ficoll-Hypaque Corning Cat. #25-072-Cl aCD3/aCD28 activating beads ThermoFisher Scientific Cat. #11132D IL-2 PeproTech Cat. #200-02 DMOG Sigma-Aldrich Cat. #D3695 Saquinavir (Protease Inhibitor) Sigma-Aldrich Cat. #S8451-50MG Raltegravir (Integrase Inhibitor) Sigma-Aldrich Cat. #CDS023737-25MG Contaminant-free PBS (for CyTOF experiments) Rockland Cat. #MB-008 EDTA (for CyTOF experiments) Corning Cat. # 46-034-CI Barcode Perm buffer Fluidigm Cat. # 201057 PFA (for CyTOF experiments) Electron Microscopy Sciences Cat. #15700 fix/perm buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #88-8824-00 Permeabilization Buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #00-8333-56 Cell-ID DNA Intercalator-Ir (for CyTOF experiments) Fluidigm Cat. #201192A Maxpar Cell Staining Buffer (for CyTOF experiments) Fluidigm Cat. # 201068 Maxpar PBS (for CyTOF experiments) Fluidigm Cat. #201058 Maxpar Cell Acquisition Solution (for CyTOF experiments) Fluidigm Cat. #201240 EQ calibration beads (for CyTOF experiments) Fluidigm Cat. #201078 retrieval solution Agilent Dako Cat. #S1700 Triton X-100 Sigma-Aldrich Cat. #X100 Zombie viability dye Biolegend Cat. #423113 live/dead staining ThermoFisher Scientific Cat. #L34961 Prolong Diamond Antifade Mount medium containing DAPI ThermoFisher Scientific Cat. #P36931 Cisplatin Sigma-Aldrich Cat. #232120-50MG Critical commercial assays NucleoSpin Plasmid, Mini kit Macherey-Nagel Cat. #740588 Plasmid Plus Maxi Kit Qiagen Cat. #12963 Quick-RNA Miniprep Kit Zymo Research Cat. #R1054 HIV-1 alliance p24 ELISA kit PerkinElmer Cat. #NEK050001KT EasySep Human CD4+ T cell Isolation Cocktail StemCell Technologies Cat. #17952 luciferase assay system Promega Cat. #E2610 nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay NanoString Technologies Cat. #121100019 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat. # 201060 PNA ISH kit Agilent Dako Cat. #K5201 RNAscope 2.5 HD Detection Reagent-RED Advanced Cell Diagnostics Cat. #322360 Deposited data NanoString nCounter data, raw and analyzed This paper GEO: GSE244193 RNA sequencing data This paper GEO: GSE244193 (Continued on next page) 18 Cell Reports 42, 113285, November 28, 2023

    Luciferase:

    Article Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P/S Fisher Scientific Cat. #11548876 Polyethylenimine Polysciences Cat. #23966 Fugene HD transfection Promega Cat. #E2311 Puromycin InvivoGen Cat. #ant-pr-1 SCH-58261 Sigma-Aldrich Cat. #S4568-5MG PMA Sigma-Aldrich Cat. #10634-1MG Ionomycin Sigma-Aldrich Cat. #10634-1MG DMSO Sigma-Aldrich Cat. #D2650-100ML Ficoll-Hypaque Corning Cat. #25-072-Cl aCD3/aCD28 activating beads ThermoFisher Scientific Cat. #11132D IL-2 PeproTech Cat. #200-02 DMOG Sigma-Aldrich Cat. #D3695 Saquinavir (Protease Inhibitor) Sigma-Aldrich Cat. #S8451-50MG Raltegravir (Integrase Inhibitor) Sigma-Aldrich Cat. #CDS023737-25MG Contaminant-free PBS (for CyTOF experiments) Rockland Cat. #MB-008 EDTA (for CyTOF experiments) Corning Cat. # 46-034-CI Barcode Perm buffer Fluidigm Cat. # 201057 PFA (for CyTOF experiments) Electron Microscopy Sciences Cat. #15700 fix/perm buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #88-8824-00 Permeabilization Buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #00-8333-56 Cell-ID DNA Intercalator-Ir (for CyTOF experiments) Fluidigm Cat. #201192A Maxpar Cell Staining Buffer (for CyTOF experiments) Fluidigm Cat. # 201068 Maxpar PBS (for CyTOF experiments) Fluidigm Cat. #201058 Maxpar Cell Acquisition Solution (for CyTOF experiments) Fluidigm Cat. #201240 EQ calibration beads (for CyTOF experiments) Fluidigm Cat. #201078 retrieval solution Agilent Dako Cat. #S1700 Triton X-100 Sigma-Aldrich Cat. #X100 Zombie viability dye Biolegend Cat. #423113 live/dead staining ThermoFisher Scientific Cat. #L34961 Prolong Diamond Antifade Mount medium containing DAPI ThermoFisher Scientific Cat. #P36931 Cisplatin Sigma-Aldrich Cat. #232120-50MG Critical commercial assays NucleoSpin Plasmid, Mini kit Macherey-Nagel Cat. #740588 Plasmid Plus Maxi Kit Qiagen Cat. #12963 Quick-RNA Miniprep Kit Zymo Research Cat. #R1054 HIV-1 alliance p24 ELISA kit PerkinElmer Cat. #NEK050001KT EasySep Human CD4+ T cell Isolation Cocktail StemCell Technologies Cat. #17952 luciferase assay system Promega Cat. #E2610 nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay NanoString Technologies Cat. #121100019 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat. # 201060 PNA ISH kit Agilent Dako Cat. #K5201 RNAscope 2.5 HD Detection Reagent-RED Advanced Cell Diagnostics Cat. #322360 Deposited data NanoString nCounter data, raw and analyzed This paper GEO: GSE244193 RNA sequencing data This paper GEO: GSE244193 (Continued on next page) 18 Cell Reports 42, 113285, November 28, 2023

    In Situ Hybridization:

    Article Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P/S Fisher Scientific Cat. #11548876 Polyethylenimine Polysciences Cat. #23966 Fugene HD transfection Promega Cat. #E2311 Puromycin InvivoGen Cat. #ant-pr-1 SCH-58261 Sigma-Aldrich Cat. #S4568-5MG PMA Sigma-Aldrich Cat. #10634-1MG Ionomycin Sigma-Aldrich Cat. #10634-1MG DMSO Sigma-Aldrich Cat. #D2650-100ML Ficoll-Hypaque Corning Cat. #25-072-Cl aCD3/aCD28 activating beads ThermoFisher Scientific Cat. #11132D IL-2 PeproTech Cat. #200-02 DMOG Sigma-Aldrich Cat. #D3695 Saquinavir (Protease Inhibitor) Sigma-Aldrich Cat. #S8451-50MG Raltegravir (Integrase Inhibitor) Sigma-Aldrich Cat. #CDS023737-25MG Contaminant-free PBS (for CyTOF experiments) Rockland Cat. #MB-008 EDTA (for CyTOF experiments) Corning Cat. # 46-034-CI Barcode Perm buffer Fluidigm Cat. # 201057 PFA (for CyTOF experiments) Electron Microscopy Sciences Cat. #15700 fix/perm buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #88-8824-00 Permeabilization Buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #00-8333-56 Cell-ID DNA Intercalator-Ir (for CyTOF experiments) Fluidigm Cat. #201192A Maxpar Cell Staining Buffer (for CyTOF experiments) Fluidigm Cat. # 201068 Maxpar PBS (for CyTOF experiments) Fluidigm Cat. #201058 Maxpar Cell Acquisition Solution (for CyTOF experiments) Fluidigm Cat. #201240 EQ calibration beads (for CyTOF experiments) Fluidigm Cat. #201078 retrieval solution Agilent Dako Cat. #S1700 Triton X-100 Sigma-Aldrich Cat. #X100 Zombie viability dye Biolegend Cat. #423113 live/dead staining ThermoFisher Scientific Cat. #L34961 Prolong Diamond Antifade Mount medium containing DAPI ThermoFisher Scientific Cat. #P36931 Cisplatin Sigma-Aldrich Cat. #232120-50MG Critical commercial assays NucleoSpin Plasmid, Mini kit Macherey-Nagel Cat. #740588 Plasmid Plus Maxi Kit Qiagen Cat. #12963 Quick-RNA Miniprep Kit Zymo Research Cat. #R1054 HIV-1 alliance p24 ELISA kit PerkinElmer Cat. #NEK050001KT EasySep Human CD4+ T cell Isolation Cocktail StemCell Technologies Cat. #17952 luciferase assay system Promega Cat. #E2610 nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay NanoString Technologies Cat. #121100019 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat. # 201060 PNA ISH kit Agilent Dako Cat. #K5201 RNAscope 2.5 HD Detection Reagent-RED Advanced Cell Diagnostics Cat. #322360 Deposited data NanoString nCounter data, raw and analyzed This paper GEO: GSE244193 RNA sequencing data This paper GEO: GSE244193 (Continued on next page) 18 Cell Reports 42, 113285, November 28, 2023

    RNAscope:

    Article Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P/S Fisher Scientific Cat. #11548876 Polyethylenimine Polysciences Cat. #23966 Fugene HD transfection Promega Cat. #E2311 Puromycin InvivoGen Cat. #ant-pr-1 SCH-58261 Sigma-Aldrich Cat. #S4568-5MG PMA Sigma-Aldrich Cat. #10634-1MG Ionomycin Sigma-Aldrich Cat. #10634-1MG DMSO Sigma-Aldrich Cat. #D2650-100ML Ficoll-Hypaque Corning Cat. #25-072-Cl aCD3/aCD28 activating beads ThermoFisher Scientific Cat. #11132D IL-2 PeproTech Cat. #200-02 DMOG Sigma-Aldrich Cat. #D3695 Saquinavir (Protease Inhibitor) Sigma-Aldrich Cat. #S8451-50MG Raltegravir (Integrase Inhibitor) Sigma-Aldrich Cat. #CDS023737-25MG Contaminant-free PBS (for CyTOF experiments) Rockland Cat. #MB-008 EDTA (for CyTOF experiments) Corning Cat. # 46-034-CI Barcode Perm buffer Fluidigm Cat. # 201057 PFA (for CyTOF experiments) Electron Microscopy Sciences Cat. #15700 fix/perm buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #88-8824-00 Permeabilization Buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #00-8333-56 Cell-ID DNA Intercalator-Ir (for CyTOF experiments) Fluidigm Cat. #201192A Maxpar Cell Staining Buffer (for CyTOF experiments) Fluidigm Cat. # 201068 Maxpar PBS (for CyTOF experiments) Fluidigm Cat. #201058 Maxpar Cell Acquisition Solution (for CyTOF experiments) Fluidigm Cat. #201240 EQ calibration beads (for CyTOF experiments) Fluidigm Cat. #201078 retrieval solution Agilent Dako Cat. #S1700 Triton X-100 Sigma-Aldrich Cat. #X100 Zombie viability dye Biolegend Cat. #423113 live/dead staining ThermoFisher Scientific Cat. #L34961 Prolong Diamond Antifade Mount medium containing DAPI ThermoFisher Scientific Cat. #P36931 Cisplatin Sigma-Aldrich Cat. #232120-50MG Critical commercial assays NucleoSpin Plasmid, Mini kit Macherey-Nagel Cat. #740588 Plasmid Plus Maxi Kit Qiagen Cat. #12963 Quick-RNA Miniprep Kit Zymo Research Cat. #R1054 HIV-1 alliance p24 ELISA kit PerkinElmer Cat. #NEK050001KT EasySep Human CD4+ T cell Isolation Cocktail StemCell Technologies Cat. #17952 luciferase assay system Promega Cat. #E2610 nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay NanoString Technologies Cat. #121100019 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat. # 201060 PNA ISH kit Agilent Dako Cat. #K5201 RNAscope 2.5 HD Detection Reagent-RED Advanced Cell Diagnostics Cat. #322360 Deposited data NanoString nCounter data, raw and analyzed This paper GEO: GSE244193 RNA sequencing data This paper GEO: GSE244193 (Continued on next page) 18 Cell Reports 42, 113285, November 28, 2023

    RNA Sequencing:

    Article Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER P/S Fisher Scientific Cat. #11548876 Polyethylenimine Polysciences Cat. #23966 Fugene HD transfection Promega Cat. #E2311 Puromycin InvivoGen Cat. #ant-pr-1 SCH-58261 Sigma-Aldrich Cat. #S4568-5MG PMA Sigma-Aldrich Cat. #10634-1MG Ionomycin Sigma-Aldrich Cat. #10634-1MG DMSO Sigma-Aldrich Cat. #D2650-100ML Ficoll-Hypaque Corning Cat. #25-072-Cl aCD3/aCD28 activating beads ThermoFisher Scientific Cat. #11132D IL-2 PeproTech Cat. #200-02 DMOG Sigma-Aldrich Cat. #D3695 Saquinavir (Protease Inhibitor) Sigma-Aldrich Cat. #S8451-50MG Raltegravir (Integrase Inhibitor) Sigma-Aldrich Cat. #CDS023737-25MG Contaminant-free PBS (for CyTOF experiments) Rockland Cat. #MB-008 EDTA (for CyTOF experiments) Corning Cat. # 46-034-CI Barcode Perm buffer Fluidigm Cat. # 201057 PFA (for CyTOF experiments) Electron Microscopy Sciences Cat. #15700 fix/perm buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #88-8824-00 Permeabilization Buffer (for CyTOF experiments) eBioscience, ThermoFisher Scientific Cat. #00-8333-56 Cell-ID DNA Intercalator-Ir (for CyTOF experiments) Fluidigm Cat. #201192A Maxpar Cell Staining Buffer (for CyTOF experiments) Fluidigm Cat. # 201068 Maxpar PBS (for CyTOF experiments) Fluidigm Cat. #201058 Maxpar Cell Acquisition Solution (for CyTOF experiments) Fluidigm Cat. #201240 EQ calibration beads (for CyTOF experiments) Fluidigm Cat. #201078 retrieval solution Agilent Dako Cat. #S1700 Triton X-100 Sigma-Aldrich Cat. #X100 Zombie viability dye Biolegend Cat. #423113 live/dead staining ThermoFisher Scientific Cat. #L34961 Prolong Diamond Antifade Mount medium containing DAPI ThermoFisher Scientific Cat. #P36931 Cisplatin Sigma-Aldrich Cat. #232120-50MG Critical commercial assays NucleoSpin Plasmid, Mini kit Macherey-Nagel Cat. #740588 Plasmid Plus Maxi Kit Qiagen Cat. #12963 Quick-RNA Miniprep Kit Zymo Research Cat. #R1054 HIV-1 alliance p24 ELISA kit PerkinElmer Cat. #NEK050001KT EasySep Human CD4+ T cell Isolation Cocktail StemCell Technologies Cat. #17952 luciferase assay system Promega Cat. #E2610 nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay NanoString Technologies Cat. #121100019 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat. # 201060 PNA ISH kit Agilent Dako Cat. #K5201 RNAscope 2.5 HD Detection Reagent-RED Advanced Cell Diagnostics Cat. #322360 Deposited data NanoString nCounter data, raw and analyzed This paper GEO: GSE244193 RNA sequencing data This paper GEO: GSE244193 (Continued on next page) 18 Cell Reports 42, 113285, November 28, 2023



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